LOCUS       V005019                 6134 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005019
VERSION     V005019
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Wada T, Noda M, Kashiwabara F, Jeon HJ, Shirakawa A, Yabu H, Matoba
            Y, Kumagai T, Sugiyama M.
  TITLE     Characterization of four plasmids harboured in a Lactobacillus
            brevis strain encoding a novel bacteriocin, brevicin 925A, and
            construction of a shuttle vector for lactic acid bacteria and
            Escherichia coli
  JOURNAL   Microbiology (Reading, Engl.) 155 (PT 5), 1726-1737 (2009)
   PUBMED   19372160
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Noda M, Sugiyama M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2007) Contact:Masafumi Noda Hiroshima University,
            Graduate School of Biomedical Sciences; Kasumi 1-2-3, Minami-ku,
            Hiroshima 734-8553, Japan
REFERENCE   3  (bases 1 to 6134)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6134)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 155 (PT 5), 1726-1737 (2009)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-DEC-2007) Contact:Masafumi Noda Hiroshima University, Graduate
            School of Biomedical Sciences; Kasumi 1-2-3, Minami-ku, Hiroshima
            734-8553, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             299..1033
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     primer_bind     1576..1592
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1593..1649
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1662..1678)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1686..1702)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1710..1740)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1755..1776)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2064..2652)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2826..3683)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3684..3788)
                     /label="AmpR promoter"
     CDS             complement(4357..5280)
                     /codon_start=1
                     /gene="repA"
                     /product="plasmid replication protein"
                     /label="repA"
                     /protein_id="BAH56454.1"
                     /translation="MSNELVKYQPELNTIPLRKFSPTEMNLFFSIISRMRDKGDQTVRF
                     SFDQLKDLSNYKPTANRRFIDDLKRTYNHLMDLRFGSQSKSGLSFERFVMFTKFKINGD
                     VEEPYVDIEIYKDALPLLNHLESWVRYALVEFRDLKSSYAKTMFRLLKGYRTTGYAYFS
                     KSDFIELLDIPKSYRQSNTDQYVISPIKEELTPLFRGLTVRKKYGKGRGKPVIGYSFAW
                     KPERKDAEDVHVSKTERLKKAKFNIEHNGELSDKEKWRAIDKIKGLKLGTTEAEHNKQE
                     QEAREEQIRAEERQKILEKLRNGGLR"
     gene            complement(4357..5280)
                     /gene="repA"
                     /label="repA"
     rep_origin      complement(5336..5509)
                     /direction=LEFT
                     /note="replication origin in Gram-positive bacteria (iteron
                     region)"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgta gaagcaaact taagagtgtg ttgatagtgc agtatcttaa aattttgtat
      241 aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gtgattacat
      301 gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat
      361 aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg
      421 gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag
      481 tcatctattc aacttatcgt cagaaaaatt aaaactgaat actcgtgtca ctttaattca
      541 ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat
      601 tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga
      661 catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact
      721 agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg
      781 ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga
      841 tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga
      901 atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa
      961 caatttaagt accgttactt atgagcaagt attgtctatt tttaatagtt atctattatt
     1021 taacgggagg aaataattct atgagtcgct tttgtaaatt tggaaagtta cacgttacta
     1081 aagggaatgt agataaatta ttaggtatac tactgacagc ttccaaggag ctaaagaggt
     1141 ccctagcggg ggatctaggc gccttgcacc ataaaattgt aaacgttaat attttgttaa
     1201 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaatagacc gaaatcggca
     1261 aaatccctta taaatcaaaa gaatagcccg agatagagtt gagtgttgtt ccagtttgga
     1321 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     1381 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     1441 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     1501 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     1561 cccagtcacg acgttgtaaa acgacggcca gtgaattcga gctcggtacc cggggatcct
     1621 ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1681 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1741 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1801 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1861 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1921 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1981 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2041 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2101 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2161 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2221 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2281 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2401 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2461 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2521 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2581 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2641 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2701 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2761 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2821 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2881 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2941 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3001 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3061 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3121 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3181 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3241 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3301 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3361 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3421 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3481 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3541 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3601 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3661 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3721 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3781 gttccgcgca catttccccg aaaagtgcca cctgacgtca gtctgcctat tcctgcttga
     3841 aaagaggggg gcttcgctcc ccccttttat ccccccctaa aaccaaagtc aaaagcatgc
     3901 cgatttcgct tcgcaaaacg gcacaaattc aagcccgaaa acctgtcctt tttttgcaaa
     3961 aaaaggacac aaaaaaaacc gcacaaaaaa agccgaaagt ttgctgttcg caaatcggct
     4021 ttttaaggca ttatgattgc tcattttctg tttgaaactg actcaaccat tggctaacat
     4081 ctttcaagtc ttcgattttc cagaagttgc cgttataagt catagcatta gcaatgtttt
     4141 gtagttgctg aaattgttct gtagttaaag ttggtgaagc cgtttctctt ttttcaaaaa
     4201 ctggcgtatg gtcgagcata ttttcggctg tagttatatt tttagcgcca gtttttttgt
     4261 aaaatttacg gccaagcttc tcatattcct tcgctagtgc cttgcgctta cgcactttca
     4321 gtttcttttc tagtttcgca atacgttcta attcggtcat cgtaatcctc cgtttcgtag
     4381 tttttcgagt attttttgtc gctcctctgc acgaatctgt tcttcgcgcg cctcttgttc
     4441 ttgtttatta tgttccgctt cggtcgttcc tagcttgaga cctttgattt tatcaattgc
     4501 ccgccatttt tctttgtcgg acagttcgcc attgtgctcg atattgaatt tggctttctt
     4561 caggcgttca gtcttgctga cgtggacatc ttcggcatct ttgcgttcag gtttccatgc
     4621 aaaagaatag ccaataacag gctttcctcg ccctttaccg tatttcttgc ggacagttag
     4681 cccacgaaat aggggggtaa gttcttcttt gattggacta ataacatatt ggtcggtgtt
     4741 agactgccgg taactttttg gaatatcaag caattcaata aaatcggatt ttgaaaaata
     4801 ggcgtaaccg gtggtgcgat aacctttcaa caaacggaac atcgttttag catagctgct
     4861 cttcaagtca cgaaactcga ctagggcgta tcgtacccag ctctccagat ggtttagcaa
     4921 gggaagtgca tctttgtaaa tctctatgtc aacatatggt tcttccacat caccgttgat
     4981 tttaaatttg gtaaacatga caaatctttc aaatgacaac ccgcttttac tttggctacc
     5041 gaaacgcaag tccatcaaat gattgtaagt gcgcttgagg tcgtcaataa atcggcgatt
     5101 ggcggtcggt ttgtagttac taaggtcttt cagctggtca aagctaaagc gtacagtctg
     5161 gtcgccctta tcccgcatac gactaatgat cgaaaagaac agattcattt ctgtaggact
     5221 gaatttacgg agtggaatgg tgttcagttc gggttgatat ttcactaact cattgctcat
     5281 ataatcacct caaaattatt atagcatata taaaaggatt tcatgcccaa attaacatgc
     5341 ctttaaacag acaattccat gcctttaaac agacaattcc atgcctttaa acagacaatt
     5401 ccatgccttt aaacagacaa ttccatgcct taatcatcct gtaccccttg ggagagtaag
     5461 gtagaacacc cccttaaaag aggttttaaa agaggtttta aaagaggtta tataagaggc
     5521 ggggatctcc gacttcgtct ccgtgcccca aaatgccaaa tcaggaccaa aaaccaaaaa
     5581 ggagtgagcg aaaatcacgc tcacattttg tttgcagcgt tcacttcgtt cactcgctaa
     5641 attcaaaatc aaaagcgtgg ttcaggaaaa gcctcgactg acgctcggca ggtaagccca
     5701 gataggcata aaaggccata ctttgcgttc taagctcgtt tggcgacttc ttcgtcgtgc
     5761 tctttttggt tttaggcaag cagttcattt gctagacaat taggtatcgc tacgctccct
     5821 aattgtctgg caaatggctt gtaagaaggg cgctacgctc ccctcttaac tccccacaaa
     5881 aatagccgat tacctatttg gcaacatcta tgaaaagcga tcgatgttgc cttgtcaatc
     5941 aactagatgt gacaatcggt gccccttctt caccaaaaat tttggtacgt taatttgcaa
     6001 atttaattaa ttagatatag aatttaatcg aacatatgtt cgtataatta tgtagcgaca
     6061 gacggacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6121 gaggcccttt cgtc
//
